Document 0524
 DOCN  M9480524
 TI    Demonstration of human T lymphotropic virus type I (HTLV-I)-specific T
       cell responses from seronegative and polymerase chain reaction-negative
       persons exposed to HTLV-I.
 DT    9410
 AU    Nishimura M; Kermode AG; Clerici M; Shearer GM; Berzofsky JA; Uchiyama
       T; Wiktor SZ; Pate E; Maloney B; Manns A; et al; Neuroimmunology Branch,
       National Institute of Neurological; Disorders and Stroke, Bethesda, MD
       20892.
 SO    J Infect Dis. 1994 Aug;170(2):334-8. Unique Identifier : AIDSLINE
       MED/94308598
 AB    Human T lymphotropic virus type I (HTLV-I) is a human retrovirus
       etiologically linked to adult T cell leukemia and the progressive
       chronic neurologic disease HTLV-I-associated myelopathy/tropical spastic
       paraparesis. Described is a method that measures the production of
       interleukin-2 from HTLV-I synthetic peptide-stimulated peripheral blood
       lymphocytes (PBL) of HTLV-I-infected persons. The peptides correspond to
       immunogenic regions of the HTLV-I Env and Tax proteins. Significantly,
       this assay demonstrated T cell responses to these HTLV-I peptides from
       coded PBL samples in 7 of 19 HTLV-I-seronegative polymerase chain
       reaction-negative persons known to have been exposed to HTLV-I but in
       none of 16 matched controls without risk factors for exposure (P =
       .007). The implications of this finding are discussed.
 DE    Adult  Amino Acid Sequence  Child, Preschool  Cohort Studies  Female
       Gene Products, env/CHEMISTRY/IMMUNOLOGY  Gene Products,
       tax/CHEMISTRY/IMMUNOLOGY  Human  HTLV-I/*IMMUNOLOGY  HTLV-I
       Antigens/CHEMISTRY/IMMUNOLOGY  HTLV-I Infections/*TRANSMISSION  Infant
       Interleukin-2/*BIOSYNTHESIS  Molecular Sequence Data  Polymerase Chain
       Reaction  Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY  JOURNAL
       ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).